Expression host
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HEK293 (Human embryonic kidney cell line). |
Purity
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Purity of adiponectin mouse recombinant is >98% (HPLC and SDS PAGE analyzed). |
Formulation
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Filtered (0.4um) and lyophilized from 0.5 mg/ml in PBS buffer. |
Solubility
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It is recommended to reconstitute the lyophilized Acrp30 Mouse in sterile 18M-cm H2O at 0.5mg/ml, which can then be further diluted to other aqueous solutions. |
Synonyms
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Acrp30, AdipoQ, GBP-28, APM-1, ACDC. |
Stability
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Store lyophilized APM-1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C. |
Amino acid sequence
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EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DYKDDDDK. |
Biological Activity
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Full-length mouse adiponectin activates AMP-activated protein kinase in hepatocyte and activates AMPK in HepG2 human hepatocytes at a concentration of 1000ng/ml corresponding to a Specific Activity of 1000IU/mg. Mouse adiponectin mammalian cell derived inhibits glucose production as shown by in-vitro gluconeogenesis assay in primary rat hepatocytes. |