Actin Antibody

Catalog Number: ASA-B0030

Lead time: 1 week

$299.00

Qty:

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
actin, gamma 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Actin(365-375aa SGPSIVHRKCF), identical to the related mouse and rat sequences.

Properties

Form	Liquid
Size	100 µg/vial
Contents	Antibody is lyophilized with 50% glycerol, 0.9 mg NaCl, 0.2 mg Na2HPO4. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied, at 2-8 °C for one month. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	ACTG1
Protein	Actin, cytoplasmic 2
Uniprot ID	P63261
Function	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Tissue Specificity
Sub-cellular localization	Cytoplasm, cytoskeleton.
Sequence Similarities	Belongs to the actin family.
Aliases	Gamma-actin

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Rat Mouse	AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information

Anti-Actin antibody, ASA-B0030, Western blotting
Lane 1: Rat Cardiac Muscle Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Rat Testis Tissue Lysate
Lane 4: Rat Skeletal Muscle Tissue Lysate
Lane 5: MM231 Cell Lysate
Lane 6: HELA Cell Lysate
Lane 7: SMMC Cell Lysate
Lane 8: HT1080 Cell Lysate
Lane 9: SW620 Cell Lysate

Anti-Actin antibody, ASA-B0030, IHC(P)
Rat Cardiac Muscle Tissue

Actin Antibody

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification

Immunogen

Properties

Form

Size

Contents

Concentration

Storage

Additional Information Regarding the Antigen

Gene

Protein

Uniprot ID

Function

Tissue Specificity

Sub-cellular localization

Sequence Similarities

Aliases

Application Details