AIF Antibody

Catalog Number: ASA-B0055

Lead time: 1 week

$299.00

Qty:

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
apoptosis-inducing factor, mitochondrion-associated, 1	Polyclonal	IgG	Rabbit	Human, Mouse, Rat	IHC-P, IHC-F, ICC, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human AIF(596-613aa EQHEDLNEVAKLFNIHED), identical to the related rat and mouse sequences.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 ml sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	AIFM1
Protein	Apoptosis-inducing factor 1, mitochondrial
Uniprot ID	O95831
Function	Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase- independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.
Tissue Specificity	Detected in muscle and skin fibroblasts (at protein level). Isoform 5 is frequently down-regulated in human cancers.
Sub-cellular localization	Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note: Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region.
Sequence Similarities	Belongs to the FAD-dependent oxidoreductase family.
Aliases	AIFM1 antibody\|Apoptosis inducing factor 1, mitochondrial antibody\|Apoptosis inducing factor antibody\|Apoptosis inducing factor, mitochondrion associated, 1 antibody\|Apoptosis-inducing factor 1 antibody\|CMTX4 antibody\|COXPD6 antibody\|Harlequin antibody\|Hq antibody\|mAIF antibody\|MGC111425 antibody\|MGC5706 antibody\|mitochondrial antibody\|PDCD 8 antibody\|PDCD8 antibody\|Programmed cell death 8(apoptosis inducing factor) antibody\|Programmed cell death 8 antibody\|Programmed cell death 8 isoform 1 antibody\|Programmed cell death 8 isoform 2 antibody\|Programmed cell death 8 isoform 3 antibody\|Programmed cell death 8 isoform 5 antibody\|Programmed cell death protein 8 antibody\|Programmed cell death protein 8 mitochondrial antibody\|Programmed cell death protein 8 mitochondrial precursor antibody\|Striatal apoptosis inducing factor antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Human, Mouse, Rat	AssaySolution's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Human, Rat Mouse	AssaySolution's IHC/ICC Detection kit
Immunohistochemistry(Frozen Section)	0.5-1μg/ml	Rat Human, Mouse	AssaySolution's IHC/ICC Detection kit
Immunocytochemistry	0.5-1μg/ml	Human Mouse, Rat	AssaySolution's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) IHC(F) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information

Anti-AIF antibody, ASA-B0055, IHC(P)
Human Lung Cancer Tissue

Anti-AIF antibody, ASA-B0055, IHC(P)
Rat Cardiac Muscle Tissue

Anti-AIF antibody, ASA-B0055, Western blotting
Lane 1: Rat Heart Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: K562 Cell Lysate
Lane 4: HEPG2 Cell Lysate
Lane 5: A431 Cell Lysate
Lane 6: NIH3T3 Cell Lysate

AIF Antibody

Overview

Long Name

Antibody Type

Antibody Isotype

Host

Species Reactivity

Validated Applications

Purification

Immunogen

Properties

Form

Size

Contents

Concentration

Storage

Additional Information Regarding the Antigen

Gene

Protein

Uniprot ID

Function

Tissue Specificity

Sub-cellular localization

Sequence Similarities

Aliases

Application Details