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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
491320 |
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Gene Symbol
|
NGAL |
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Species
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Canine |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Dog NGAL . No significant cross-reactivity or interference between Dog NGAL and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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6.25 -- 400 pg/ml |
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Sensitivity
|
5 pg/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Dog NGAL ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by
analogy with its homologues in other mammalian species, be released from activated neutrophils in
inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells
in response to ischemic or nephrotoxic injury.
(NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It
is expressed by neutrophils and various epithelial cells, including tubular cells of the kidney. It is involved
in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It
is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and
certain adenocarcinomas.
and siderocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic
molecules. 24p3 was observed to be dramatically upregulated in kidney cell cultures infected with SV40
and subsequently identified as a lipocalin. Unlike human NGAL, it is an acute phase protein upregulated
in the liver. but like its mammalian homologues it is upregulated and secreted by the kidney in response
to ischemic or nephrotoxic injury. It is also upregulated in the involuting uterus and mammary gland. |
