|
Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
|
Gene ID
|
11096 |
|
Gene Symbol
|
ADAMTS5 |
|
Synonym
|
A disintegrin and metalloproteinase with thrombospondin motifs 11; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 5 (aggrecanase-2); ADAM metallopeptidase with thrombospondin type 1 motif, 5; ADAM-TS 11; ADAM-TS 5; ADAMTS11; ADAMTS-11; ADAMTS5; ADAM-TS5; ADAMTS-5; ADMP2; ADMP-2; ADMP-2A disintegrin and metalloproteinase with thrombospondin motifs 5; Aggrecanase 2; aggrecanase-2; disintegrin-like and metalloprotease with thrombospondin type 1 motif, 510ADAMTS11FLJ36738; EC 3.4.24; EC 3.4.24.-; EC 3.4.24.14; EC 3.4.24.82 |
|
Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for Human ADAMTS5. No significant cross-reactivity or Human ADAMTS5 and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
|
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Standard Curve Range
|
125--8000 pg/ml |
|
Sensitivity
|
100 pg/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
|
Typical Data
|
ELISA: Human ADAMTS5 ELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
ADAMTS5 is a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage. ADAMTS5 may also have a role in the pathogenesis of human osteoarthritis. |
