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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
|
173 |
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Gene Symbol
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Afamin |
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Synonym
|
Afamin; AFM; ALB2; ALB2alpha-Alb; ALBA; ALBAalpha-albumin; ALF; Alpha-Alb; Alpha-albumin; MGC125338; MGC125339 |
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Species
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Human |
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Specificity
|
This assay has high sensitivity and excellent specificity for detection of human Afamin. No significant cross-reactivity or interference between human Afamin and analogues was observed. |
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Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
78.1 pg/ml - 5000 pg/ml |
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Sensitivity
|
62.5 pg/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human Afamin ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Afamin is a protein that in humans is encoded by the AFM gene. This gene is a member of the albumin gene family, which comprises four genes that localize to chromosome 4 in a tandem arrangement. These four genes encode structurally related serum transport proteins that are known to be evolutionarily related. The protein encoded by this gene is regulated developmentally, expressed in the liver and secreted into the bloodstream. |
