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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
718 |
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Gene Symbol
|
C3A |
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Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human C3a/C3a(desArg)/C3 . No significant cross-reactivity or interference between Human C3a/C3a(desArg)/C3 and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
156.25 -- 10000 pg/ml |
|
Sensitivity
|
125 pg/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
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Typical Data
|
ELISA: Human C3a/C3a(desArg)/C3 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Complement C3a is an anaphylatoxin of 77 amino acid residues released by the action of the C3
convertases on the N-terminal of the alpha chain of C3. It is rapidly inactivated by serum
carboxypeptidase N which removes the C-terminal arginine residue generating C3a (desArg). |
