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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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6360 |
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Gene Symbol
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CCL16 |
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Synonym
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C-C motif chemokine 16; CCL16; chemokine (C-C motif) ligand 16; Chemokine CC-4; Chemokine LEC; CKb12; HCC4; HCC-4; HCC-4Liver-expressed chemokine; LCC-1Lymphocyte and monocyte chemoattractant; LEC; liver CC chemokine-1; LMC; LMCSmall-inducible cytokine A16; monotactin-1; Mtn-1; NCC-4; NCC4IL-10-inducible chemokine; NCC-4ILINCK; new CC chemokine 4; SCYA16MGC117051; SCYL4; small inducible cytokine subfamily A (Cys-Cys), member 16 |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human CCL16/HCC-4. No significant cross-reactivity or interference between human CCL16/HCC-4 and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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7.81 pg/ml - 500 pg/ml |
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Sensitivity
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7.5 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human CCL16/HCC-4 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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Chemokine (C-C motif) ligand 16 (CCL16) is a small cytokine belonging to the CC chemokine family that is known under several pseudonyms, including Liver-expressed chemokine (LEC) and Monotactin-1 (MTN-1). This chemokine is expressed by the liver, thymus, and spleen and is chemoattractive for monocytes and lymphocytes. Cellular expression of CCL16 can be strongly induced in monocytes by IL-10, IFN-γ and bacterial lipopolysaccharide. Its gene is located on chromosome 17, in humans, among a cluster of other CC chemokines. CCL16 elicits its effects on cells by interacting with cell surface chemokine receptors such as CCR1, CCR2, CCR5 and CCR8. C-C motif chemokine ligand 16 has been found in high levels in the blood plasma of humans. CCL16 may be useful for trafficking eosinophils. This ligand has been found to have a functional affinity for H4 receptors that are expressed by eosinophils and mast cells. This chemokine has been shown to suppress rapid proliferation of myeloid progenitor cells. |
