Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
|
Gene ID
|
943 |
Gene Symbol
|
CD30 |
Synonym
|
CD30 antigen; CD30; CD30KI-1; CD30L receptor; cytokine receptor CD30; D1S166EKi-1; Ki-1 antigen; Lymphocyte activation antigen CD30; TNFRSF8; tumor necrosis factor receptor superfamily member 8; tumor necrosis factor receptor superfamily, member 8 |
Species
|
Human |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of human CD30/TNFRSF8. No significant cross-reactivity or interference between human CD30/TNFRSF8 and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
93.8 pg/ml - 6000 pg/ml |
Sensitivity
|
75 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Human CD30/TNFRSF8 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is expressed by activated, but not by resting, T and B cells. TRAF2 and TRAF5 can interact with this receptor, and mediate the signal transduction that leads to the activation of NF-kappaB. It is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. |