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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
720 |
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Gene Symbol
|
C4C |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Human Complement component C4c . No significant cross-reactivity or interference between Human Complement component C4c and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
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Typical Data
|
ELISA: Human Complement component C4c ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
C4c is a component in the complement cascade and is a degradation product of C4b which is cleaved by
C4b/C3b inactivator to yield C4d and C4c (soluble). C4c consists of a g-chain (30 kDa), a b-chain (75 kDa),
and two a-domains denoted a3 (27 kDa) and a4 (14 kDa). C4c reacts with C1 and C2 to form C3
convertase of the classic activation pathway |
