|
Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
|
Gene ID
|
1675 |
|
Gene Symbol
|
CFD |
|
Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Complement factor D . No significant cross-reactivity or interference between Human Complement factor D and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
|
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Standard Curve Range
|
250 -- 16000 pg/ml |
|
Sensitivity
|
200 pg/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
|
Typical Data
|
ELISA: Human Complement factor D ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Complement factor D is a serine protease of the alternative pathway of complement activation. The
protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to
C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment. |
