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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
5199 |
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Gene Symbol
|
CFP |
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Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Properdin . No significant cross-reactivity or interference between Human Properdin and analogues was observed. |
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Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
|
Typical Data
|
ELISA: Human Properdin ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Properdin in plasma is a mixture of cyclic dimers, trimers and tetramers. The molecular weight of the
glycosylated monomer is 53 kDa. Properdin is a regulator protein of the alternative complement pathway.
It stabilizes the C3 convertase excerting its effect by binding to C3b in the C3bBb complex and thereby
inhibiting cleavage of C3b by factor I and increasing the affinity for factor B. Serum concentration is
approximately 25 μg/mL. |
