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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
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Gene ID
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5657 |
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Gene Symbol
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PRTN3 |
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Synonym
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ACPA; AGP7; AGP7PR3; azurophil granule protein 7; C-ANCA; EC 3.4.21; EC 3.4.21.76; Leukocyte proteinase 3; MBN; MBT; Myeloblastin; Neutrophil proteinase 4; NP4; NP-4; P29C-ANCA antigen; PR-3CANCA; Proteinase 3; PRTN3; serine proteinase, neutrophil; Wegener autoantigen; Wegener granulomatosis autoantigen |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of human Proteinase 3/PRTN3. No significant cross-reactivity or interference between human Proteinase 3/PRTN3 and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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15.6 pg/ml - 1000 pg/ml |
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Sensitivity
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12.5 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human Proteinase 3/PRTN3 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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Proteinase 3 also known as PRTN3 is an enzyme that in humans is encoded by the PRTN3 gene. PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown, but, in human neutrophils, proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the target of anti-neutrophil cytoplasmic antibodies (ANCAs) of the c-ANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease granulomatosis with polyangiitis (formerly known as "Wegener's granulomatosis"). |
