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Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
|
2147 |
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Gene Symbol
|
TAT |
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Species
|
Human |
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Specificity
|
This assay has high sensitivity and excellent specificity for detection of Human Thrombin-antithrombin(TAT) . No significant cross-reactivity or interference between Human Thrombin-antithrombin(TAT) and analogues was observed. |
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Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
|
100 - 6250 pg/ml |
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Sensitivity
|
80 pg/ml |
|
Inter Assay
|
CV%<10% |
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Intra Assay
|
CV%<8% |
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Assay Type
|
Sandwich ELISA |
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Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
|
50-100ul |
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Applications
|
ELISA |
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Typical Data
|
ELISA: Human Thrombin-antithrombin(TAT) ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
|
Thrombin (Factor IIa) is an enzyme, a serine protease, which participates in blood clotting. It is formed
when prothrombin (Factor II) is proteolytically cleaved to form thrombin, which is glycoprotein formed by two
peptides chains of 36 and 259 amino acids. Thrombin converts soluble fibrinogen into insoluble strands of
fibrin and is then neutralized by antithrombin in order to for a thrombin-antithrombin (TAT) complex. |
