|
Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
|
|
Gene ID
|
7056 |
|
Gene Symbol
|
BDCA-3 |
|
Synonym
|
BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM |
|
Species
|
Human |
|
Specificity
|
This assay has high sensitivity and excellent specificity for detection of human Thrombomodulin/BDCA-3. No significant cross-reactivity or interference between human Thrombomodulin/BDCA-3 and analogues was observed. |
|
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
|
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
|
Standard Curve Range
|
31.3 pg/ml - 2000 pg/ml |
|
Sensitivity
|
25 pg/ml |
|
Inter Assay
|
CV%<10% |
|
Intra Assay
|
CV%<8% |
|
Assay Type
|
Sandwich ELISA |
|
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
|
Sample Volume
|
50-100ul |
|
Applications
|
ELISA |
|
Typical Data
|
ELISA: Human Thrombomodulin/BDCA-3 ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
Background
|
Thrombomodulin (TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor (TAFI, aka carboxypeptidase B2) into its active form. |
