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Storage
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Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
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Gene ID
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332 |
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Gene Symbol
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API4 |
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Synonym
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API4 survivin variant 3 alpha; API4; Apoptosis inhibitor 4 baculoviral IAP repeat-containing protein 5; Apoptosis inhibitor survivin; baculoviral IAP repeat-containing 5; BIRC5; EPR-1; IAP4; Survivin |
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Species
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Human |
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Specificity
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This assay has high sensitivity and excellent specificity for detection of Human Total Survivin . No significant cross-reactivity or interference between Human Total Survivin and analogues was observed. |
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Kit Components
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Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
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Notes
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Please contact our Technical Services with any questions regarding species reactivity |
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Standard Curve Range
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62.5--4000 pg/ml |
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Sensitivity
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50 pg/ml |
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Inter Assay
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CV%<10% |
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Intra Assay
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CV%<8% |
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Assay Type
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Sandwich ELISA |
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Suitable Sample Type
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serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
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Sample Volume
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50-100ul |
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Applications
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ELISA |
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Typical Data
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ELISA: Human Total Survivin ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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Background
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Survivin is a member of the inhibitor of apoptosis (IAP) family. The survivin protein functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. This has been shown by disruption of survivin induction pathways leading to increase in apoptosis and decrease in tumour growth. The survivin protein is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells. These data suggest survivin might provide a new target for cancer therapy that would discriminate between transformed and normal cells. Survivin expression is also highly regulated by the cell cycle and is only expressed in the G2-M phase. It is known that Survivin localizes to the mitotic spindle by interaction with tubulin during mitosis and may play a contributing role in regulating mitosis. The molecular mechanisms of survivin regulation are still not well understood, but regulation of survivin seems to be linked to the p53 protein. It also is a direct target gene of the Wnt pathway and is upregulated by beta-catenin. |
