Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past
expiration date.
|
Gene ID
|
563 |
Gene Symbol
|
ZAG |
Synonym
|
alpha-2-glycoprotein 1, zinc; alpha-2-glycoprotein 1, zinc-binding; AZGP1; ZA2G; ZAG; ZAGzn-alpha-2-GP; zinc; zinc-alpha-2-glycoprotein; zn-alpha-2-glycoprotein; ZNGP1 |
Species
|
Human |
Specificity
|
This assay has high sensitivity and excellent specificity for detection of human ZAG. No significant cross-reactivity or interference between human ZAG and analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
62.5 pg/ml - 4000 pg/ml |
Sensitivity
|
50 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Human ZAG ELISA Kit (Colorimetric) - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
Zinc-alpha-2-glycoprotein is a protein that in humans is encoded by the AZGP1 gene. This gene expresses a soluble protein that stimulates lipolysis, induces a reduction in body fat in mice, is associated with the cachexia related to cancer, and is known to be expressed in secretory cells of lung epithelium. In 2009, it was found that smoking increases expression of this gene, which is why smoking cessation leads to weight gain. Zinc-alpha-2-glycoprotein levels also rise with onset of Diabetes 2, which accounts for weight loss thereafter. |