Storage
|
Store the unopened product at 2 - 8° C. Protect from light. Do not use past expiration date. |
Gene ID
|
18606 |
Gene Symbol
|
ENPP2 |
Synonym
|
ATX; ATXFLJ26803; ATX-X; Autotaxin; autotaxin-t; EC 3.1.4.39; ectonucleotide pyrophosphatase/phosphodiesterase 2; ectonucleotide pyrophosphatase/phosphodiesterase family member 2; E-NPP 2; ENPP2; ENPP-2; Extracellular lysophospholipase D; Lysophosphatidic Acid; LysoPLD; NPP2; PD-IALPHA; PDNP2; PDNP2NPP2; phosphodiesterase I/nucleotide pyrophosphatase 2; plasma lysophospholipase D |
Species
|
Mouse |
Specificity
|
This assay has high sensitivity and excellent specificity for Mouse ENPP-2/Autotaxin. No significant cross-reactivity or Mouse ENPP-2/Autotaxinand analogues was observed. |
Kit Components
|
Assay plate (12 x 8 coated Microwells), Standard (Freeze dried), Biotin-antibody (60 x concentrate), HRP-avidin (20 x concentrate), Biotin-antibody Diluent, HRP-avidin Diluent, Sample Diluent, Wash Buffer (20 x concentrate), TMB Substrate, Stop Solution, Adhesive Strip (For 96 wells), Instruction manual |
Notes
|
Please contact our Technical Services with any questions regarding species reactivity |
Standard Curve Range
|
78.1--5000 pg/ml |
Sensitivity
|
62.5 pg/ml |
Inter Assay
|
CV%<10% |
Intra Assay
|
CV%<8% |
Assay Type
|
Sandwich ELISA |
Suitable Sample Type
|
serum, plasma, tissue homogenates, cell lysate, cell culture medium. |
Sample Volume
|
50-100ul |
Applications
|
ELISA |
Typical Data
|
ELISA: Mouse ENPP-2/AutotaxinELISA Kit (Colorimetric) These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
Background
|
Autotaxin, also known as ectonucleotide pyrophosphatase/phosphodiesterase 2 (NPP2 or ENPP2), is a secreted enzyme important for generating the lipid signaling molecule lysophosphatidic acid (LPA). Autotaxin has lysophospholipase D activity that converts lysophosphatidylcholine into LPA. Autotaxin was originally identified as a tumor cell-motility-stimulating factor; later it was shown to be LPA (which signals through lysophospholipid receptors), the lipid product of the reaction catalyzed by autotaxin, which is responsible for its effects on cell-proliferation. |